Role of Protein Tyrosine Phosphorylation on COX-2 Induction
Author Information
Author(s): P. Akarasereenont, J. A. Mitchell, I. Appleton, C. Thiemermann, J. R. Vane
Primary Institution: The William Harvey Research Institute, St Bartholomews' Hospital Medical College
Hypothesis
The study investigates the role of tyrosine kinase activation in the induction of COX-2 and iNOS in endotoxin-activated endothelial cells and macrophages.
Conclusion
Tyrosine phosphorylation is part of the signal transduction mechanism that mediates the induction of COX-2 and iNOS in macrophages and endothelial cells activated by endotoxin.
Supporting Evidence
- Tyrosine kinase inhibitors caused dose-dependent inhibition of COX metabolites in LPS-activated cells.
- Western blot analysis showed inhibition of COX-2 protein expression by tyrosine kinase inhibitors.
- Endotoxin activated macrophages produced nitrite, which was inhibited by the tyrosine kinase inhibitors.
Takeaway
The study shows that certain proteins help control inflammation in cells when they are activated by toxins.
Methodology
The study used tyrosine kinase inhibitors to assess their effects on COX-2 and iNOS induction in bovine aortic endothelial cells and J774.2 macrophages after endotoxin treatment.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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