New PCR Systems for Detecting Mycobacterium avium subsp. paratuberculosis
Author Information
Author(s): David Herthnek, Göran Bölske
Primary Institution: National Veterinary Institute (SVA), Department of Bacteriology, Uppsala, Sweden
Hypothesis
Can new real-time PCR systems provide reliable confirmation of Mycobacterium avium subsp. paratuberculosis detection?
Conclusion
The newly constructed DH3 PCR system on the F57 gene is recommended as the primary confirmatory test for PCR positives.
Supporting Evidence
- The confirmatory PCR systems were found sensitive and specific.
- The F57 system did not elicit any false positives.
- DNA from both naturally infected and spiked faeces could be confirmed with the new systems.
Takeaway
Scientists created new tests to check for a germ that causes a disease in cows, making sure they work well to find it.
Methodology
Two new real-time PCR systems targeting IS900 and one targeting the F57 gene were developed and validated on strains and clinical samples.
Limitations
The F57 system is slightly less sensitive than the primary IS900 system.
Digital Object Identifier (DOI)
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