The Anticancer Activity of Cannabinol (CBN) and Cannabigerol (CBG) on Acute Myeloid Leukemia Cells
Author Information
Author(s): Kadriya Ahmad, Forbes-Robertson Sarah, Falah Mizied, Calhelha Ricardo
Primary Institution: Medical Research Institute, The Holy Family Hospital Nazareth
Hypothesis
The study aimed to assess the antileukemic effects of CBN and CBG on an acute monocytic leukemia cell line, the THP-1.
Conclusion
CBN and CBG exhibited suppressive, cell death-inducing effects on leukemia cells.
Supporting Evidence
- Both CBN and CBG inhibited cell growth and induced THP-1 cell apoptosis and cell cycle arrest in a dose- and time-dependent manner.
- CBG was found to be more effective than CBN in reducing cell viability.
- At doses exceeding 20 μM, less than 50% of the THP-1 cells remained viable after 48 hours.
Takeaway
This study found that two compounds from cannabis, CBN and CBG, can help kill leukemia cells and stop them from growing.
Methodology
The study used MTT assays, flow cytometry, and Western blotting to analyze the effects of CBN and CBG on THP-1 cell viability, morphology, and p53 expression.
Limitations
The exact molecular mechanisms of CBN and CBG's effects on leukemia cells are still poorly understood.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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