Plasma L-Cystine/L-Glutamate Imbalance Increases Tumor Necrosis Factor-Alpha from CD14+ Circulating Monocytes in Patients with Advanced Cirrhosis
2011

L-Cystine Imbalance Increases Inflammatory Response in Cirrhosis Patients

Sample size: 19 publication 10 minutes Evidence: moderate

Author Information

Author(s): Kakazu Eiji, Ueno Yoshiyuki, Kondo Yasuteru, Inoue Jun, Ninomiya Masashi, Kimura Osamu, Wakui Yuta, Fukushima Koji, Tamai Keiichi, Shimosegawa Tooru

Primary Institution: Tohoku University Graduate School of Medicine

Hypothesis

How does the plasma L-Cystine/L-Glutamate imbalance affect the function of circulating monocytes in patients with advanced cirrhosis?

Conclusion

An imbalance of plasma L-Cystine and L-Glutamate in patients with advanced cirrhosis leads to increased TNF-alpha production from monocytes, indicating immune dysfunction.

Supporting Evidence

  • The addition of L-Cystine significantly increased TNF-alpha production from monocytes.
  • Monocytes from patients with advanced cirrhosis showed higher TNF-alpha mRNA levels correlated with the L-Cystine/L-Glutamate ratio.
  • High levels of extracellular L-Cystine enhanced the antiport mechanism affecting monocyte function.

Takeaway

In patients with severe liver disease, a problem with certain amino acids makes their immune cells work too hard, which can lead to more inflammation.

Methodology

The study involved measuring plasma amino acid concentrations and evaluating the function of CD14+ monocytes in patients with advanced cirrhosis.

Potential Biases

Potential bias due to the selection of patients and the specific conditions of the study.

Limitations

The study is limited by its small sample size and the need for further investigation into other immune cells.

Participant Demographics

Patients with advanced cirrhosis classified by Child-Pugh grade B or C.

Statistical Information

P-Value

0.0018

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0023402

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