HSP90 Controls SIR2 Mediated Gene Silencing
Author Information
Author(s): Laskar Shyamasree, Bhattacharyya Mrinal K., Shankar Rama, Bhattacharyya Sunanda
Primary Institution: University of Hyderabad
Hypothesis
The activity of the telomeric protein Sir2p is modulated by Hsp82, the ortholog of Hsp90 from budding yeast.
Conclusion
Hsp90 homeostasis maintains the cellular pool of Sir2p and controls the reversible nature of telomere silencing.
Supporting Evidence
- Hsp82 homeostasis plays a critical role in maintaining cellular pools of enzymatically active Sir2p.
- Over-expression of Hsp82 shows a negative regulatory effect on telomere silencing.
- Sir2p function is dependent on Hsp82, as shown in temperature-sensitive mutant strains.
Takeaway
Hsp90 helps keep a protein called Sir2p at the right level, which is important for keeping certain genes turned off at the ends of chromosomes.
Methodology
The study involved yeast strains and various assays to assess the effects of Hsp82 on Sir2p levels and gene silencing.
Limitations
The study primarily focuses on yeast models, which may not fully represent similar mechanisms in other organisms.
Digital Object Identifier (DOI)
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