Development and validation of an enzyme-linked immunosorbent assay for antibodies against Mycobacterium bovis in european wild boar
2008

Testing for Bovine Tuberculosis in Wild Boar

Sample size: 185 publication Evidence: moderate

Author Information

Author(s): Aurtenetxe Olaia, Barral Marta, Vicente Joaquín, de la Fuente José, Gortázar Christian, Juste Ramón A

Primary Institution: Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER-TECNALIA)

Hypothesis

Can an ELISA test effectively detect antibodies against Mycobacterium bovis in European wild boar?

Conclusion

The developed ELISA test shows high potential for accurately diagnosing TB in wild boar with good sensitivity and specificity.

Supporting Evidence

  • The ELISA test achieved a sensitivity of 72.60% and specificity of 96.43%.
  • The test was validated on 185 serum samples from wild boar.
  • The study indicates that wild boar can act as a reservoir for Mycobacterium bovis.

Takeaway

Scientists created a test to check if wild boars are sick with a disease called TB, and it works pretty well.

Methodology

An enzyme-linked immunosorbent assay (ELISA) was developed and validated using serum samples from wild boar with known TB status.

Limitations

Some negative group animals showed a positive reaction, indicating potential false positives.

Participant Demographics

Wild boar from Spain, including both TB positive and negative individuals.

Statistical Information

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1746-6148-4-43

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