CsmA Protein and BChl a in Chlorosomes of Oscillochloris trichoides
Author Information
Author(s): Zobova Anastasiya, Taisova Alexandra, Lukashev Eugeny, Fedorova Nataliya, Baratova Ludmila, Fetisova Zoya
Primary Institution: A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University
Hypothesis
Does the baseplate BChl a subantenna exist in Oscillochloris trichoides chlorosomes as a complex with the CsmA protein?
Conclusion
The study confirms that the baseplate BChl a subantenna exists in Oscillochloris trichoides chlorosomes as a complex with the 5.7 kDa CsmA protein.
Supporting Evidence
- The presence of BChl a was confirmed by fluorescence and absorption spectra.
- Alkaline treatment selectively removed BChl a and the CsmA protein from chlorosomes.
- Fluorescence spectra showed that BChl a emission was linked to the presence of CsmA.
Takeaway
The researchers found that a specific protein called CsmA is linked to a type of pigment in certain bacteria, helping them capture light for photosynthesis.
Methodology
The study used absorbance and fluorescence spectroscopy, along with SDS-PAGE analysis, to investigate the chlorosomes.
Digital Object Identifier (DOI)
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