Standard Operating Procedures: The Viral MetaGenome Annotation Pipeline (VMGAP): an automated tool for the functional annotation of viral Metagenomic shotgun sequencing data
2011

Viral MetaGenome Annotation Pipeline (VMGAP)

Sample size: 300000 publication Evidence: high

Author Information

Author(s): Lorenzi Hernan A., Hoover Jeff, Inman Jason, Safford Todd, Murphy Sean, Kagan Leonid, Williamson Shannon

Primary Institution: J. Craig Venter Institute

Hypothesis

The VMGAP pipeline improves the functional annotation of viral metagenomic data.

Conclusion

The VMGAP can assign functions to significantly more viral sequences compared to existing tools like MG-RAST.

Supporting Evidence

  • The VMGAP assigned almost 16% more functional names to viral sequences compared to MG-RAST.
  • VMGAP incorporates specialized databases for better functional annotation.
  • Results from VMGAP can be imported into JCVI Metagenomic Reports for further analysis.

Takeaway

The VMGAP is a tool that helps scientists understand viruses better by giving names to their genes, making it easier to study them.

Methodology

The VMGAP uses a multi-step process involving database searches and functional assignments to annotate viral metagenomic datasets.

Limitations

The pipeline's performance may be affected by the quality of input data and the availability of relevant databases.

Digital Object Identifier (DOI)

10.4056/sigs.1694706

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