Photofrin Distribution in Tumor Cells and Macrophages
Author Information
Author(s): M. Korbelik, G. Krosll, P.L. Olive, D.J. Chaplin
Primary Institution: British Columbia Cancer Research Centre
Hypothesis
Do tumor-associated macrophages accumulate more Photofrin than tumor cells?
Conclusion
Tumor-associated macrophages accumulate significantly higher levels of Photofrin compared to tumor cells.
Supporting Evidence
- Photofrin levels were consistently higher in tumor-associated macrophages than in tumor cells.
- At 4-12 hours post-administration, macrophages had up to 3 times more Photofrin than tumor cells.
- The study used fluorescence activated cell sorting to analyze the distribution of Photofrin.
Takeaway
This study found that special immune cells called macrophages take up more of a cancer drug called Photofrin than the actual cancer cells do.
Methodology
Flow cytometric analysis and cell sorting were used to measure Photofrin levels in tumor cells and macrophages from SCCVII tumors in mice.
Limitations
The study may have limitations related to the enzymatic dissociation procedure affecting Photofrin levels.
Participant Demographics
Female C3H mice aged 9-11 weeks were used in the experiments.
Statistical Information
P-Value
p<0.005
Statistical Significance
p<0.005
Want to read the original?
Access the complete publication on the publisher's website