Study of scFv Display and Expression Levels
Author Information
Author(s): Scott Nathan, Reynolds Catherine, Wright Michael J, Qazi Omar, Fairweather Neil, Deonarain Mahendra P
Primary Institution: UCB-Celltech
Hypothesis
The display level of single-chain Fv (scFv) antibodies is influenced by their overall expression levels.
Conclusion
The propensity of a scFv to be displayed on phage is directly related to its overall expression level.
Supporting Evidence
- A strong positive correlation (Rho = 0.88) was found between scFv expression and display levels.
- Display propensity varied significantly among the 15 scFv clones despite high sequence homology.
- The study suggests that factors influencing overall expression indirectly affect phage display propensity.
Takeaway
This study found that how much scFv proteins are shown on phage is closely linked to how much of those proteins are made in bacteria.
Methodology
The study used phage display to select scFvs and measured their expression levels in E. coli using western blot and ELISA.
Limitations
The study does not account for potential mis-folding or aggregation of scFvs in the periplasm.
Statistical Information
P-Value
<0.005
Statistical Significance
p<0.005
Digital Object Identifier (DOI)
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