Anti-TIM-2 Antibody Increases Arthritis Symptoms
Author Information
Author(s): Kawamoto Toshio, Abe Yoshiyuki, Ito Jun, Makino Fumihiko, Kojima Yuko, Usui Yoshihiko, Ma Juan, Morimoto Shinji, Yagita Hideo, Okumura Ko, Takasaki Yoshinari, Akiba Hisaya
Primary Institution: Juntendo University School of Medicine
Hypothesis
Does TIM-2 contribute to the development of T helper (Th) 1 or Th17 cells and joint inflammation in collagen-induced arthritis?
Conclusion
TIM-2 signaling influences B cell proliferation and antibody production during the early phase of collagen-induced arthritis, but does not affect the induction of Th1 or Th17 cells.
Supporting Evidence
- Anti-TIM-2 mAbs treatment significantly exacerbated the development of collagen-induced arthritis.
- Serum levels of anti-CII antibodies were significantly increased in anti-TIM-2-treated mice.
- TIM-2 expression was found on splenic B cells and was up-regulated by specific stimulation.
Takeaway
Researchers found that a specific antibody made arthritis worse by making B cells produce more antibodies, but it didn't change the T cells that help fight the disease.
Methodology
DBA/1 mice were treated with anti-TIM-2 mAbs during the early or late phase of collagen-induced arthritis, and various immune responses were measured.
Participant Demographics
Male DBA/1 mice, aged 7 to 10 weeks.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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