Affinity-Enriched Plasma Proteomics for Biomarker Discovery in Abdominal Aortic Aneurysms
2024

Finding Blood Markers for Abdominal Aortic Aneurysms

Sample size: 90 publication 10 minutes Evidence: high

Author Information

Author(s): Palstrøm Nicolai Bjødstrup, Nielsen Kristian Boje, Campbell Amanda Jessica, Soerensen Mette, Rasmussen Lars Melholt, Lindholt Jes Sanddal, Beck Hans Christian

Primary Institution: Odense University Hospital

Hypothesis

Can mass spectrometry-based proteomics identify potential diagnostic biomarkers for abdominal aortic aneurysms?

Conclusion

The study identified a panel of ten proteins that can predict the presence of abdominal aortic aneurysms with high accuracy.

Supporting Evidence

  • 292 proteins were significantly regulated between AAA patients and controls.
  • 10 proteins were identified as highly predictive of AAA.
  • The random forest classifier achieved an AUC of 0.93.

Takeaway

Researchers looked at blood samples from people with and without a serious condition called abdominal aortic aneurysm to find proteins that could help doctors diagnose it better.

Methodology

Mass spectrometry-based proteomics analysis of plasma samples from 45 patients with AAA and 45 matched controls.

Potential Biases

Potential confounding effects from comorbidities and medications among participants.

Limitations

The study did not analyze proteoforms and lacked experimental validation of the identified proteins.

Participant Demographics

Participants were 65-74 years old, with 45 diagnosed with AAA and 45 controls matched for age and other factors.

Statistical Information

P-Value

0.0066

Confidence Interval

95% CI: 0.91–0.95

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.3390/proteomes12040037

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