PCR Master Mixes Can Contain Mouse DNA
Author Information
Author(s): Philip W. Tuke, Kate I. Tettmar, Asif Tamuri, Jonathan P. Stoye, Richard S. Tedder
Primary Institution: National Health Service Blood and Transplant, Colindale, London, United Kingdom
Hypothesis
Can PCR master mixes harbor murine DNA sequences that lead to false positives in XMRV detection?
Conclusion
The study suggests that contamination from PCR reagents may explain the presence of murine sequences in blood samples previously thought to contain XMRV/pMLV.
Supporting Evidence
- Two blood samples tested positive for murine sequences despite previous negative results.
- Contamination was confirmed in water controls when using specific PCR reagents.
- The study highlights the importance of careful reagent selection in viral detection.
Takeaway
Sometimes, the tools scientists use to test for viruses can accidentally pick up bits of mouse DNA, which can confuse the results.
Methodology
The study tested cDNA from blood donors using various PCR techniques to check for murine DNA contamination.
Potential Biases
Potential bias due to reliance on specific PCR reagents that may contain murine DNA.
Limitations
The study primarily focused on contamination from specific PCR reagents and may not account for other sources of contamination.
Participant Demographics
Blood donors from the National Health Service Blood and Transplant center in London.
Digital Object Identifier (DOI)
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