A New Antibody Against Japanese Encephalitis and Zika Viruses
Author Information
Author(s): Chen Dong, Zhang Jiayi, Liu Yusha, Zhu Jiayang, Chen Jie, Ni Hongxia, Wen Jinsheng
Primary Institution: Ningbo University
Hypothesis
Can a human monoclonal antibody isolated from a JEV vaccine-vaccinated volunteer neutralize various flaviviruses?
Conclusion
The antibody LZY3412 effectively neutralizes both JEV and ZIKV infections in vitro and improves survival rates in infected mice.
Supporting Evidence
- mAb LZY3412 showed high binding affinity to E proteins of JEV and ZIKV.
- NT50 values for neutralizing JEV and ZIKV were 19.9 ng/mL and 631 ng/mL, respectively.
- Application of mAb LZY3412 significantly improved survival rates in neonatal mice infected with JEV or ZIKV.
Takeaway
Scientists found a special antibody that can help protect against two viruses, JEV and Zika, which can make people very sick.
Methodology
The study involved isolating B cells from a vaccinated volunteer, sequencing them, and testing the resulting antibody's ability to neutralize viruses in vitro and in vivo.
Potential Biases
Potential bias due to the small sample size and the specific population from which the antibody was derived.
Limitations
The study was conducted with a single volunteer, which may limit the generalizability of the findings.
Participant Demographics
One healthy 22-year-old volunteer who received the JEV vaccine.
Statistical Information
P-Value
0.0196
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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