Development and some applications of enzyme-linked immunosorbent assay system for murine macrophage inflammatory protein-2 (MIP-2)
1996
Development of an ELISA System for MIP-2
publication
Evidence: moderate
Author Information
Author(s): H. Ochiai, S. Sakai, T. Kogure, T. Hirabaytashi, K. Nakajima, K. Terasawa
Primary Institution: Toyama Medical and Pharmaceutical University
Hypothesis
We attempted to establish an enzyme-linked immunosorbent assay (ELISA) system for murine MIP-2.
Conclusion
The established ELISA system is a useful tool for studying MIP-2 response in various inflammation models in mice.
Supporting Evidence
- The ELISA system could detect MIP-2 at concentrations ranging from 20 to 1000 pg/ml.
- MIP-2 production was undetectable at 0 hours but increased significantly over time in LPS-stimulated cells.
- Dexamethasone inhibited MIP-2 production in a dose-dependent manner.
Takeaway
The researchers created a test to measure a protein involved in inflammation, which can help understand how the body reacts to inflammation.
Methodology
The study involved creating a recombinant MIP-2 protein and testing its detection using an ELISA system with RAW264.7 cells.
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