LIF Helps Mouse Stem Cells Stay Pluripotent by Promoting STAT3 Secretion
Author Information
Author(s): Xu Li, Ji Jinjun, Wang Lingbo, Pan Jieli, Xiao Mingzhe, Zhang Chenxi, Gan Yihong, Xie Guanqun, Tan Mingdian, Wang Xinchang, Wen Chengping, Fan Yongsheng, Chin Y. Eugene
Primary Institution: Zhejiang Chinese Medical University
Hypothesis
Does LIF promote the secretion of STAT3 through Sec15b to maintain stem cell pluripotency during mouse embryonic development?
Conclusion
LIF-induced interaction between STAT3 and Sec15b facilitates STAT3 translocation to multivesicular endosomes, regulating the balance of ERK and GSK3β pathways to maintain stem cell pluripotency.
Supporting Evidence
- LIF maintains self-renewal growth in mouse embryonic stem cells by activating STAT3.
- STAT3 translocates into multivesicular endosomes for secretion.
- Disruption of the STAT3 and Sec15b interaction leads to developmental abnormalities in mice.
Takeaway
LIF helps mouse stem cells stay in a state where they can become any type of cell by helping a protein called STAT3 move to special bubbles in the cell, which then release it.
Methodology
The study involved analyzing the translocation of STAT3 in mouse embryonic stem cells and its interaction with Sec15b, using various molecular biology techniques including Western blotting and immunofluorescence.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website