The distinct binding properties between avian/human influenza A virus NS1 and Postsynaptic density protein-95 (PSD-95), and inhibition of nitric oxide production
2011
Influenza A Virus NS1 Protein and Its Interaction with PSD-95
publication
Evidence: moderate
Author Information
Author(s): Zhang Heng, Li Weizhong, Wang Gefei, Su Yun, Zhang Chi, Chen Xiaoxuan, Xu Yanxuan, Li Kangsheng
Primary Institution: Shantou University Medical College
Hypothesis
NS1 binds to PSD-95, and this binding affects neuronal function.
Conclusion
The study indicates that NS1 of influenza A virus can bind with neuronal PSD-95, and the avian H5N1 and human H1N1 influenza A viruses possess distinct binding properties.
Supporting Evidence
- NS1 of avian influenza virus H5N1 can bind to PSD-95, while NS1 of human influenza virus H1N1 cannot.
- Binding of NS1 to PSD-95 significantly reduces nitric oxide production in rat hippocampal neurons.
- The study provides insights into the distinct binding properties of NS1 proteins from avian and human influenza A viruses.
Takeaway
The NS1 protein from the avian flu can stick to a brain protein called PSD-95, which might change how brain cells work.
Methodology
Yeast two-hybrid analysis, GST-pull down assays, and co-immunoprecipitations were used to detect the interaction between NS1 and PSD-95.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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