Effects of In Vitro and In Vivo Passage on Murine Fibrosarcomas
Author Information
Author(s): M.F.A. Woodruff, B.A. Hodson
Primary Institution: Medical Research Council Clinical and Population Cytogenetics Unit, Western General Hospital
Hypothesis
What are the effects of in vitro and in vivo passage on the tumorigenicity and immunogenicity of murine fibrosarcomas?
Conclusion
The study found that murine fibrosarcomas lose tumorigenicity when cultured in vitro but regain it after passage in immunodeficient mice.
Supporting Evidence
- Only 6 of the 46 lines tested grew in normal adult CBA mice.
- After a single passage in irradiated mice, 33 of 39 clones tested grew in normal mice.
- Both mouse-passaged and cultured cell lines possess strong TATA.
Takeaway
When cancer cells are grown in a lab, they often can't grow in normal mice, but if they are passed through special mice that lack certain immune cells, they can grow again in normal mice.
Methodology
The study involved injecting various cloned cell lines into different types of mice to assess tumorigenicity and immunogenicity.
Limitations
The study may not account for all variables affecting tumor growth and immune response.
Participant Demographics
Female CBA backcross mice were used in the experiments.
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