Validation of the Tetracycline Regulatable Gene Expression System for Studying Infectious Disease
Author Information
Author(s): Chaturvedi Ashok K., Lazzell Anna L., Saville Stephen P., Wormley Floyd L. Jr., Monteagudo Carlos, Lopez-Ribot Jose L.
Primary Institution: University of Texas at San Antonio
Hypothesis
Does doxycycline affect host responses during the pathogenesis of candidiasis?
Conclusion
Doxycycline does not significantly affect host responses during the infection process of candidiasis.
Supporting Evidence
- The presence of doxycycline did not alter survival rates in infected mice.
- Fungal burdens in organs were similar regardless of doxycycline treatment.
- Histopathological findings were virtually identical for treated and untreated groups.
Takeaway
This study shows that giving mice doxycycline doesn't change how their bodies respond to a fungal infection.
Methodology
The study used a murine model of disseminated candidiasis to assess the effects of doxycycline on host immune responses.
Limitations
Results may not apply to mucosal models of infection where antibiotics could impact normal bacteria.
Participant Demographics
6–8 week old female BALB/c mice
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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