Role of Tissue Transglutaminase 2 in Cancer Cell Invasion
Author Information
Author(s): Lin Chun-Yu, Tsai Pei-Hsun, Kandaswami Chithan C, Chang Geen-Dong, Cheng Chia-Hsiung, Huang Chang-Jen, Lee Ping-Ping, Hwang Jiuan-Jiuan, Lee Ming-Ting
Primary Institution: Institute of Biochemical Sciences, National Taiwan University
Hypothesis
This study explores the molecular mechanisms associated with TG2's involvement in the acquisition of the mesenchymal phenotype using highly invasive A431 tumor cells.
Conclusion
TG2 activates PI3K/Akt and NF-κB signaling systems, which induce Snail and MMP-9 expression, facilitating the acquisition of a mesenchymal phenotype.
Supporting Evidence
- A431-III cells showed increased TG2 expression and enhanced mesenchymal characteristics.
- Knockdown of TG2 reduced cell motility and expression of mesenchymal markers.
- Overexpression of TG2 in A431-P cells induced EMT-like changes.
Takeaway
The study shows that a protein called TG2 helps cancer cells change into a more aggressive form, making them better at spreading in the body.
Methodology
The study used A431 tumor cell lines, transfected them with TG2 siRNA, and assessed changes in cell behavior and signaling pathways.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website