Isoproterenol-induced changes in perfusion, function, energy metabolism and nitric oxide pathway: in vivo and ex vivo study in the rat heart
2011

Effects of Isoproterenol on Heart Function and Structure in Rats

Sample size: 28 publication Evidence: moderate

Author Information

Author(s): Desrois Martine, Kober Frank, Lan Carole, Dalmasso Christiane, Cole Mark A, Clarke Kieran, Cozzone Patrick J, Bernard Monique

Primary Institution: CRMBM UMR CNRS 6612, Marseille, France

Hypothesis

Isoproterenol induces changes in myocardial perfusion, function, energy metabolism, and nitric oxide pathway in the rat heart.

Conclusion

Isoproterenol increases myocardial perfusion and induces morphological changes in the heart within the first 24 hours, leading to cardiac hypertrophy and decreased function.

Supporting Evidence

  • Left ventricular mass increased in the isoproterenol group after 1 day.
  • Diastolic wall thickness peaked at day 2 in the isoproterenol group.
  • Myocardial blood flow increased at day 1 in the isoproterenol group.
  • Cardiac function in explanted hearts was reduced by 36% in the isoproterenol group after 7 days.
  • PCr/ATP ratio was lower in the isoproterenol group compared to control.
  • MDA content was higher in the isoproterenol group compared to control.
  • eNOS expression and total nitrate concentration were increased in the isoproterenol group.

Takeaway

When rats were given a drug called isoproterenol, their hearts changed in size and function quickly, which helps us understand heart problems better.

Methodology

Male Wistar rats were infused with isoproterenol or vehicle for 7 days, and cardiac function and perfusion were assessed using MRI and biochemical assays.

Participant Demographics

Male Wistar rats

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1532-429X-13-S1-P348

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