Genomic Variation in Burkholderia pseudomallei
Author Information
Author(s): Tumapa Sarinna, Holden Matthew TG, Vesaratchavest Mongkol, Wuthiekanun Vanaporn, Limmathurotsakul Direk, Chierakul Wirongrong, Feil Edward J, Currie Bart J, Day Nicholas PJ, Nierman William C, Peacock Sharon J
Primary Institution: Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Hypothesis
Does the variation in genomic islands in Burkholderia pseudomallei affect its virulence and environmental survival?
Conclusion
Horizontal gene transfer of mobile genetic elements can rapidly alter the gene repertoire of B. pseudomallei.
Supporting Evidence
- The presence of genomic islands did not differ between environmental and disease-associated isolates.
- The cumulative number of genomic islands per isolate ranged from 0 to 5, with a median of 2.
- No significant association was found between the presence of genomic islands and clinical features in patients with melioidosis.
Takeaway
This study looked at how different parts of the bacteria's DNA can change and affect its ability to make people sick, but found no clear link between these changes and disease.
Methodology
In silico analysis of genome sequences and multiplex PCR screening of isolates.
Limitations
The study did not find significant associations between genomic islands and clinical features.
Participant Demographics
186 isolates from northeast Thailand, including 83 environmental and 103 clinical isolates.
Statistical Information
Statistical Significance
p > 0.05
Digital Object Identifier (DOI)
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