B Cell Receptor Signaling and Internalization Are Mutually Exclusive Events
Author Information
Author(s): Hou Ping, Araujo Elizabeth, Zhao Tong, Zhang Miao, Massenburg Don, Veselits Margaret, Doyle Colleen, Dinner Aaron R, Clark Marcus R
Primary Institution: University of Chicago
Hypothesis
The relationship between B cell antigen receptor signaling and internalization is unknown.
Conclusion
Phosphorylated B cell receptors are retained on the cell surface, while non-phosphorylated receptors are rapidly internalized.
Supporting Evidence
- Phosphorylated BCR complexes are preferentially retained on the cell surface.
- Non-phosphorylated BCRs are rapidly endocytosed.
- Mathematical modeling indicates that competition between phosphorylation and internalization enhances signaling responses to low avidity ligands.
Takeaway
When B cells recognize something, they can either send a signal or take in the invader, but they can't do both at the same time.
Methodology
The study used biochemical and confocal microscopy techniques to analyze B cell receptor behavior.
Limitations
The study did not explore all potential mechanisms of receptor internalization.
Digital Object Identifier (DOI)
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