HuR's Role in Tumor Endothelial Cells and Angiogenesis
Author Information
Author(s): Kurosu T, Ohga N, Hida Y, Maishi N, Akiyama K, Kakuguchi W, Kuroshima T, Kondo M, Akino T, Totsuka Y, Shindoh M, Higashino F, Hida K
Primary Institution: Department of Vascular Biology, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan
Hypothesis
HuR is associated with the angiogenic phenotype of tumor endothelial cells (TECs).
Conclusion
HuR is crucial for the upregulation of VEGF-A and COX-2 mRNA in TECs, maintaining their angiogenic properties.
Supporting Evidence
- HuR protein accumulates in the cytoplasm of TECs but not in normal endothelial cells.
- VEGF-A and COX-2 mRNA levels decrease with HuR knockdown.
- HuR knockdown inhibits cell survival, motility, and tube formation in TECs.
- VEGF and PGE2 can restore some functions in HuR-knockdown TECs.
Takeaway
HuR helps keep certain messages in cells that make blood vessels grow, which is important for tumors.
Methodology
TECs and normal endothelial cells were isolated, and the effects of HuR knockdown on mRNA levels and cellular functions were evaluated.
Statistical Information
P-Value
<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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