How Defective Ribosomes Are Recognized in Budding Yeast
Author Information
Author(s): Leporé Nathalie, Lafontaine Denis L. J.
Primary Institution: Université Libre de Bruxelles
Hypothesis
How ribosomal RNA synthesis, pre-rRNA processing, and nucleolar surveillance are integrated is unclear.
Conclusion
The study indicates that the Nrd1-Nab3 complex contributes to recruiting nucleolar surveillance to elongating polymerases to survey nascent rRNA transcripts.
Supporting Evidence
- The interaction between the transcription elongation factor Spt5 and Rpa190 requires the Spt5 C-terminal region.
- Mutations in the RNA binding domain of Nrd1 led to the accumulation of normal and aberrant polyadenylated pre-rRNAs.
- Nrd1-Nab3 contributes to recruiting nucleolar surveillance to elongating polymerases.
- The Spt4-Spt5 complex influences RNA Pol I transcription both positively and negatively.
Takeaway
This study looks at how cells recognize and deal with faulty ribosomes, which are important for making proteins. It finds that certain proteins help monitor and fix problems with ribosome production.
Methodology
The study used yeast genetics, chromatin immunoprecipitation, and Northern blotting to analyze the interactions and functions of various RNA processing factors.
Statistical Information
P-Value
0.0039
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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