Developing and Mapping Genetic Markers in Oak Trees
Author Information
Author(s): Durand Jérôme, Bodénès Catherine, Chancerel Emilie, Frigerio Jean-Marc, Vendramin Giovanni, Sebastiani Federico, Buonamici Anna, Gailing Oliver, Koelewijn Hans-Peter, Villani Fiorella, Mattioni Claudia, Cherubini Marcello, Goicoechea Pablo G, Herrán Ana, Ikaran Ziortza, Cabané Cyril, Ueno Saneyoshi, Alberto Florian, Dumoulin Pierre-Yves, Guichoux Erwan, de Daruvar Antoine, Kremer Antoine, Plomion Christophe
Primary Institution: INRA, UMR1202 BIOGECO, F-33610 Cestas, France
Hypothesis
Can expressed sequence tags (ESTs) be effectively used to develop microsatellite markers for oak species?
Conclusion
The study successfully generated a bin map for oak that includes 256 EST-SSRs, aiding in the establishment of a gene-based map for this genus.
Supporting Evidence
- A total of 103,000 Sanger ESTs were assembled into 28,024 unigenes.
- 18.6% of the unigenes presented one or more SSR motifs.
- 37.7% of the designed primer pairs amplified a single polymorphic locus.
Takeaway
Researchers found a way to create markers that help study oak trees by looking at their genes. This will help scientists understand how these trees adapt to their environment.
Methodology
The study involved mining expressed sequence tags (ESTs) for simple sequence repeats (SSRs) and using a bin mapping approach to assign these markers to a genetic map.
Limitations
The level of polymorphism in EST-SSRs was found to be lower than that of genomic SSRs.
Digital Object Identifier (DOI)
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