New Method to Measure Tissue Factor and Thrombomodulin Activities
Author Information
Author(s): Frederix Kim, Kooter Ingeborg M, van Oerle René, Fens Diane, Hamulyak Karly, Gerlofs-Nijland Miriam E, ten Cate Hugo, Spronk Henri MH
Primary Institution: Maastricht University
Hypothesis
Exposure to particulate matter stimulates blood coagulation by an indirect inflammation mediated pathway, disturbing the tissue factor/thrombomodulin balance.
Conclusion
Inflammation associated procoagulant effects in tissues depend on variations in the activity of the tissue factor-thrombomodulin balance.
Supporting Evidence
- Inflammation increased tissue factor activity in the lungs of endotoxemic animals.
- Thrombin generation was significantly higher in lung homogenates from LPS-treated mice compared to controls.
- Particulate matter exposure led to increased tissue factor activity and decreased thrombomodulin activity in rat lungs.
Takeaway
This study found that inflammation can change how blood clots in different tissues, which might help us understand diseases caused by pollution.
Methodology
The study developed a new functional assay to assess tissue-specific procoagulant activity using thrombin generation curves from tissue homogenates added to human plasma.
Limitations
The study did not specify which cell types express the observed pro- or anticoagulant activities.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
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