Combined Xanthorrhizol-Curcumin Effect on Breast Cancer Cells
Author Information
Author(s): Cheah Yew Hoong, Nordin Fariza Juliana, Sarip Rozie, Tee Thiam Tsui, Azimahtol Hawariah Lope Pihie, Sirat Hasnah M, Rashid Badrul Amini Abd, Abdullah Noor Rain, Ismail Zakiah
Primary Institution: Institute for Medical Research, Kuala Lumpur, Malaysia
Hypothesis
The combination of xanthorrhizol and curcumin might show synergistic growth inhibitory effect towards MDA-MB-231 human breast cancer cells via apoptosis induction.
Conclusion
The combined cytotoxic effect of xanthorrhizol and curcumin on MDA-MB-231 cells has been documented, showing synergistic growth inhibitory activity via apoptosis induction.
Supporting Evidence
- Isobole analysis revealed that the interaction of xanthorrhizol and curcumin was synergistic when added concurrently.
- Immunofluorescence staining indicated altered mitochondrial transmembrane potential, a hallmark of apoptosis.
- DNA fragmentation assay confirmed apoptotic cell death induced by the treatment.
Takeaway
Mixing two natural substances, xanthorrhizol and curcumin, can help stop the growth of breast cancer cells by making them die in a special way called apoptosis.
Methodology
The study used sulforhodamine B (SRB) assay to determine the GI50 and isobolographic analysis to evaluate the synergistic activity of xanthorrhizol and curcumin.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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