cDNA Immunization of Mice with Human Thyroglobulin Generates Both Humoral and T Cell Responses
Author Information
Author(s): Jacobson Eric M., Concepcion Erlinda, Ho Kenneth, Kopp Peter, Vono Toniolo Jussara, Tomer Yaron
Primary Institution: University of Cincinnati College of Medicine
Hypothesis
Can cDNA immunization with human thyroglobulin generate a model of thyroid autoimmunity in mice?
Conclusion
The study developed a novel mouse model of thyroid autoimmunity using cDNA immunization that generates both B and T cell responses without the need for adjuvants.
Supporting Evidence
- Electroporation significantly increased the levels of Tg reactive antibodies compared to direct delivery.
- Mice immunized with hTg cDNA developed both B and T cell responses.
- The model allows for testing of various hypotheses regarding thyroid autoimmunity.
Takeaway
Researchers found a way to make mice's immune systems react to a human protein, which helps us understand thyroid diseases better.
Methodology
Mice were immunized with a plasmid cDNA encoding human thyroglobulin and electroporated to enhance immune response.
Limitations
The model did not show lymphocytic infiltration of the thyroid, which may limit its relevance to human disease.
Participant Demographics
Female C3H/Hen mice, 8 weeks old.
Statistical Information
P-Value
0.037
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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