3D Gene Expression and Cell Fate in Chick Wing
Author Information
Author(s): Fisher Malcolm, Downie Helen, Welten Monique C. M., Delgado Irene, Bain Andrew, Planzer Thorsten, Sherman Adrian, Sang Helen, Tickle Cheryll
Primary Institution: University of Dundee
Hypothesis
Which transcription factor genes are involved in specifying digit identity in the developing chick wing?
Conclusion
The study reveals that specific transcription factor genes play distinct roles in digit identity during different stages of chick wing development.
Supporting Evidence
- Tbx2 and Tbx3 are expressed in digit 3 and 4 progenitors at all stages.
- Hoxd13 and Sall1 expression is dynamic, changing with development stages.
- Sox9 expression in digit condensations aligns with digit progenitor domains.
Takeaway
This study looks at how certain genes help decide what each finger will be in a chick's wing as it grows.
Methodology
The researchers used Optical Projection Tomography to capture 3D gene expression patterns and fate mapping in chick wing development.
Limitations
The analysis was conducted at the transcript level, and protein expression patterns may differ.
Digital Object Identifier (DOI)
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