Metabolic Response of Tumor-Bearing Mice to Fasting
Author Information
Author(s): A.M. Rofe, S.J. Porter, R. Bais, R.A.J. Conyers
Primary Institution: Metabolic Research Group, Division of Clinical Chemistry, Institute of Medical and Veterinary Science
Hypothesis
The ketonaemic response to fasting may be altered in the tumour-bearing state.
Conclusion
Fasted tumor-bearing mice show an initial increase in ketonaemia that is not sustained due to diminished lipid reserves.
Supporting Evidence
- Ketone body concentrations in the blood of the tumour-bearing mice were significantly increased after a 24h fast compared to control mice.
- Hepatic glycogen levels were lower at the start of the fasting period in the tumour-bearing mice.
- The loss of adipose tissue during the fasting period was greater in the tumour-bearing mice.
- After 48 h of fasting, ketonaemia was significantly lower in the tumour-bearing mice compared to the appropriate controls.
Takeaway
When mice with tumors fast, they initially produce more ketones, but they run out of fat stores faster than healthy mice, leading to less ketone production later.
Methodology
Male C57/BL6j mice were fasted and their blood and liver metabolites were measured at 2, 24, and 48 hours after the last meal.
Limitations
The study may underestimate fat loss due to potential fluid retention in tumor-bearing mice.
Participant Demographics
Male C57/BL6j mice, 12-16 weeks of age.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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