CSF Lipids and Neuroinflammation in Multiple Sclerosis
Author Information
Author(s): Shi Lisa, Ghezzi Laura, Fenoglio Chiara, Pietroboni Anna Margherita, Galimberti Daniela, Pace Francesca, Hardy Todd A, Piccio Laura, Don Anthony S
Primary Institution: The University of Sydney
Hypothesis
The CSF lipidome will differ substantially in demyelinating diseases such as MS and NMOSD compared with other neuroinflammatory diseases.
Conclusion
CSF sphingolipids are positively correlated with markers of neuroinflammation and differentiate NMOSD from MS.
Supporting Evidence
- Twenty-five sphingolipids and four lysophosphatidylcholines were significantly higher in NMOSD compared with MS.
- A combination of three sphingolipids differentiated NMOSD from MS with an area under the curve of 0.92.
- Ninety-four lipids were positively correlated with macrophage migration inhibitory factor (MIF).
- EDSS was inversely correlated with cholesterol ester CE(16:0) in both MS cohorts.
Takeaway
This study found that certain fats in the fluid around the brain can help tell the difference between two diseases, MS and NMOSD, and might help doctors understand how sick someone is.
Methodology
Lipids and inflammatory cytokines were quantified in CSF from people with untreated MS, NMOSD, and other neurological diseases using liquid chromatography-tandem mass spectrometry and multiplex ELISA.
Potential Biases
Potential bias due to the absence of healthy controls and limited range of EDSS scores.
Limitations
The study had a low sample size for NMOSD and did not include healthy controls.
Participant Demographics
Participants included 33 MS cases, 11 NMOSD cases, and others with inflammatory and non-inflammatory neurological diseases.
Statistical Information
P-Value
p<0.0025
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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