Detecting Cancer Cells in Pelvic Lavage After Rectal Cancer Surgery
Author Information
Author(s): Kristensen Annette Torgunrud, Wiig Johan N, Larsen Stein G, Giercksky Karl-Erik, Ekstrøm Per O
Primary Institution: Rikshospitalet-Radiumhospitalet Medical Center, University of Oslo
Hypothesis
The presence of k-ras mutated cells in pelvic lavage after rectal cancer surgery is a prognostic factor.
Conclusion
Patients with k-ras mutated cells in the lavage immediately after surgery have a reduced life expectation.
Supporting Evidence
- 19 out of 237 rectal cancer patients had positive lavage fluid.
- Patients with k-ras positive lavage had a mean survival of 22 months compared to 47 months for k-ras negative patients.
- Only N- and R-stage were significantly different between k-ras positive and negative groups.
Takeaway
This study found that some patients have cancer cells in their belly after surgery, and those patients tend to live shorter lives.
Methodology
DNA from cells obtained by lavage was analyzed for mutations in the k-ras gene using denaturing capillary electrophoresis.
Limitations
This is a pilot study and results need confirmation in larger studies.
Participant Demographics
Patients included 59% men and 41% women, aged 29 to 87 years with a median age of 66.
Statistical Information
P-Value
0.006
Confidence Interval
C.I 14.6–29.1 for k-ras positive; C.I 43.2–50.8 for k-ras negative
Statistical Significance
p = 0.006
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website