Stromal Influences on Breast Cancer Cell Growth
Author Information
Author(s): C.E.P. van Roozendaal, B. van Ooijen, J.G.M. Klijn, C. Claassen, A.M.M. Eggermont, S.C. Henzen-Logmans, J.A. Foekens
Primary Institution: Dr Daniel den Hoed Cancer Center
Hypothesis
Paracrine influences from fibroblasts derived from different sources of breast tissue affect epithelial breast cancer cell growth in vitro.
Conclusion
Stromal factors play a role in the growth regulation of human breast cancer cells, with effects varying depending on the source of the stroma and the characteristics of the epithelial tumor cells.
Supporting Evidence
- Conditioned media from fibroblasts derived from breast tumor tissue significantly stimulated the growth of MCF-7 cells.
- MCF-7 cells showed a higher proliferation index with conditioned media from tumor-derived fibroblasts compared to adjacent normal tissue.
- Skin fibroblast conditioned media caused a high range proliferation index similar to tumor-derived fibroblasts.
Takeaway
Fibroblasts from different types of breast tissue can help or hurt the growth of breast cancer cells, depending on where they come from.
Methodology
Fibroblasts were grown from surgically removed breast or skin tissues and their conditioned media were tested for effects on breast cancer cell lines.
Limitations
The study does not clarify the qualitative or quantitative variations in the secreted growth factors from different fibroblast sources.
Participant Demographics
Fibroblasts were derived from malignant breast tumors, adjacent normal breast tissue, normal breast tissues from reduction mammoplasties, and skin tissue.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Want to read the original?
Access the complete publication on the publisher's website