Astrocyte-Derived Tissue Transglutaminase and Its Role in Astrocyte Adhesion and Migration
Author Information
Author(s): van Strien Miriam E., Brevé John J. P., Fratantoni Silvina, Schreurs Marco W. J., Bol John G. J. M., Jongenelen Cornelis A. M., Drukarch Benjamin, van Dam Anne-Marie
Primary Institution: VU University Medical Center, Neuroscience Campus Amsterdam, Amsterdam, The Netherlands
Hypothesis
TG2 is involved in astrocyte-fibronectin interactions.
Conclusion
Astrocyte-derived TG2 contributes to the interaction between astrocytes and fibronectin, potentially regulating ECM remodeling and glial scarring.
Supporting Evidence
- TG2 expression and activity increased after treatment with pro-inflammatory cytokines.
- Active TG2 on the surface of astrocytes enhances adhesion to fibronectin.
- Inhibition of TG2 activity reduces astrocyte adhesion and migration.
- Astrocyte-derived TG2 interacts with fibronectin, contributing to ECM remodeling.
- Cytokine treatment alters astrocyte migration capacity.
Takeaway
This study shows that a protein called TG2 helps brain cells called astrocytes stick to a special protein called fibronectin, which is important for healing in the brain.
Methodology
Primary rat astrocytes were treated with cytokines and analyzed for TG2 expression, adhesion, and migration on fibronectin.
Potential Biases
Potential bias in interpreting results due to the use of specific inhibitors and cytokine treatments.
Limitations
The study primarily uses rat astrocytes, which may not fully represent human astrocyte behavior.
Participant Demographics
Newborn Dark Agouti rats were used for astrocyte cultures.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website