Protooncogene Amplification in Non-Small Cell Lung Cancer
Author Information
Author(s): R.J.C. Slebos, S.G. Evers, S.S. Wagenaar, S. Rodenhuis
Primary Institution: The Netherlands Cancer Institute
Hypothesis
Can protooncogene abnormalities contribute to the classification of non-small cell lung cancer?
Conclusion
Amplification of protooncogenes is a rare event in non-small cell lung cancer.
Supporting Evidence
- Two adenocarcinomas showed a 5-7 fold amplification of the c-myc gene.
- One adenocarcinoma showed a 3-5 fold amplification of the H-ras gene.
- K-ras and neu gene amplifications were found in lung metastases from colorectal and breast cancer respectively.
- None of the tumors with an amplified protooncogene had a mutationally activated K-ras gene.
Takeaway
This study looked at lung cancer samples to see if certain genes were copied more than usual, but found that this doesn't happen very often.
Methodology
The study analyzed 52 lung tumor specimens and four cell lines for protooncogene amplification using Southern blotting.
Limitations
The study focused only on specific protooncogenes and may not represent all genetic alterations in lung cancer.
Participant Demographics
The study included 48 patients with various types of non-small cell lung cancer.
Want to read the original?
Access the complete publication on the publisher's website