DNA Preservation in Hypersaline Lakes
Author Information
Author(s): Borin Sara, Crotti Elena, Mapelli Francesca, Tamagnini Isabella, Corselli Cesare, Daffonchio Daniele
Primary Institution: University of Milan
Hypothesis
The study aims to investigate the persistence of extracellular DNA and its biological transforming activity in extreme environments like the deep hypersaline anoxic lakes of the Mediterranean Sea.
Conclusion
Naked DNA was demonstrated to be preserved and biologically active in these extreme environments, potentially serving as a genetic reservoir for horizontal gene transfer.
Supporting Evidence
- Extracellular DNA was preserved for 32 days in axenic conditions.
- Plasmid DNA maintained its capacity to transform naturally competent cells.
- The Discovery lake was the most aggressive environment toward living cells.
- Different brines had varying effects on the conformation of plasmid DNA.
- Halobacillus trueperi was the most resistant strain, surviving up to 144 days.
Takeaway
In super salty lakes, DNA from dead cells can stick around for a long time and still work, which means it can be shared with other living cells.
Methodology
Seven strains of bacteria were isolated from the seawater-brine interface and incubated in hypersaline brines to study their survival and the preservation of plasmid DNA.
Limitations
The presence of naturally competent bacteria should be demonstrated, and the role of filterable agents like DNAses and viruses needs further evaluation.
Statistical Information
P-Value
p<0.03
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website