Endostatin expression in a pancreatic cell line is modulated by a TNFα-dependent elastase
2005
How TNFα Affects Endostatin in Pancreatic Cells
publication
Evidence: moderate
Author Information
Author(s): Brammer R D, Bramhall S R, Eggo M C
Primary Institution: University of Birmingham
Hypothesis
We hypothesized that endostatin production could be modulated by protease activity under the influence of TNFα.
Conclusion
Endostatin is released by SUIT-2 cells, and increases in intracellular elastase, induced by TNFα, are correlated with increased secretion.
Supporting Evidence
- Endostatin is produced by SUIT-2 cells but not by BxPc-3 cells.
- TNFα increases the secretion of endostatin in a dose-dependent manner.
- Endostatin is susceptible to degradation by intracellular proteases.
Takeaway
This study found that a substance called endostatin, which helps control blood vessel growth, is produced more when a certain protein (TNFα) is present in pancreatic cancer cells.
Methodology
The study used cell culture, RT-PCR, and Western blotting to analyze endostatin production and degradation in pancreatic cell lines.
Digital Object Identifier (DOI)
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