Oxidative Stress Increases Fas Ligand Expression in Endothelial Cells
Author Information
Author(s): Suzuki Mayumi, Aoshiba Kazutetsu, Nagai Atsushi
Primary Institution: Tokyo Women's Medical University
Hypothesis
Does oxidative stress from H2O2 and cigarette smoke increase Fas ligand expression in endothelial cells?
Conclusion
Low levels of oxidative stress increase FasL expression on endothelial cells, potentially reducing leukocyte extravasation and tissue damage.
Supporting Evidence
- HUVECs exposed to H2O2 showed increased membrane FasL expression.
- Cigarette smoke extracts also increased FasL expression in endothelial cells.
- HUVECs exposed to H2O2 promoted neutrophil apoptosis.
- Rat thoracic aortas incubated with H2O2 exhibited increased FasL expression.
Takeaway
When cells are exposed to small amounts of stress from things like hydrogen peroxide or cigarette smoke, they make more Fas ligand, which helps keep harmful immune cells from causing damage.
Methodology
Human umbilical vein endothelial cells were exposed to H2O2 and cigarette smoke extracts, and FasL expression was measured using immunostaining and flow cytometry.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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