Genomic Survey of the Varroa Destructor Mite
Author Information
Author(s): Scott R Cornman, Michael C Schatz, Spencer J Johnston, Yan-Ping Chen, Jeff Pettis, Greg Hunt, Lanie Bourgeois, Chris Elsik, Denis Anderson, Christina M Grozinger, Jay D Evans
Primary Institution: USDA-ARS, Bee Research Laboratory
Hypothesis
How can understanding the genome of Varroa destructor advance mite control strategies?
Conclusion
The genomic survey provides tools for research and new directions for controlling Varroa mites.
Supporting Evidence
- The genome size was estimated to be 565 Mbp, larger than most sequenced insects.
- Genomic DNA pooled from ~1,000 mites was sequenced to 4.3× coverage.
- Identified over 13,000 contigs with sequences homologous to other species.
Takeaway
Scientists studied the DNA of a tiny pest that harms honey bees to find new ways to help the bees stay healthy.
Methodology
The genome was sequenced using flow cytometry and 454 pyrosequencing, analyzing DNA from approximately 1,000 mites.
Potential Biases
Potential contamination from associated microbes during DNA extraction.
Limitations
The assembly is fragmented and may not capture the full coding potential of Varroa destructor.
Participant Demographics
Mites collected from multiple honey bee colonies.
Statistical Information
P-Value
6.2 × 10-5
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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