Comparison of four different colorimetric and fluorometric cytotoxicity assays in a zebrafish liver cell line
2008
Comparing Cytotoxicity Assays in Zebrafish Liver Cells
publication
Evidence: moderate
Author Information
Author(s): Stephanie K Bopp, Teresa Lettieri
Primary Institution: European Commission – Joint Research Centre
Hypothesis
Which cytotoxicity assay is most effective for zebrafish liver cell line ZFL?
Conclusion
The alamarBlue and CFDA-AM assays are recommended for cytotoxicity assessment in ZFL cells.
Supporting Evidence
- All four assays performed well in the ZFL cells.
- Effective concentrations causing 10% or 50% loss of cell viability varied by a maximum factor of 7.0 for EC10 values.
- The LDH assay showed the highest intra- and inter-assay variabilities.
- The MTT assay requires cell destruction, while AB and CFDA-AM are non-destructive.
Takeaway
Scientists tested four different ways to see how toxic substances affect zebrafish liver cells and found two methods that work best.
Methodology
Four cytotoxicity assays were compared using the zebrafish liver cell line ZFL and four model compounds.
Limitations
No effective concentrations could be determined for Benzo[a]pyrene due to incomplete dose-response curves.
Statistical Information
P-Value
p<0.05
Confidence Interval
95%
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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