Detection of protein catalytic residues at high precision using local network properties
2008

Detecting Protein Catalytic Residues Using Local Network Properties

Sample size: 226 publication 10 minutes Evidence: high

Author Information

Author(s): Patrick Slama, Ioannis Filippis, Michael Lappe

Primary Institution: Max Planck Institute for Molecular Genetics

Hypothesis

Can local network descriptors derived from protein structures effectively identify catalytic residues in enzymes?

Conclusion

The proposed method significantly improves the detection of catalytic residues in proteins, achieving a precision of 28.1% and a higher precision of 72.7% for functional residues.

Supporting Evidence

  • The method achieved a precision of 28.1% for detecting catalytic residues.
  • Precision for functional residues reached 72.7% on a smaller validation set.
  • The scoring function outperformed existing methods that rely on closeness centrality and residue surface accessibility.

Takeaway

This study introduces a new way to find important parts of proteins that help them work, which can be useful for designing new medicines.

Methodology

The study used residue interaction networks derived from protein structures to evaluate local network parameters for detecting catalytic residues.

Potential Biases

Potential bias in the selection of proteins and the definition of catalytic residues.

Limitations

The method's performance may vary with different protein families and structures.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2105-9-517

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