Vaccinia virus A12L protein and its AG/A proteolysis play an important role in viral morphogenic transition
2007
Role of A12L Protein in Vaccinia Virus Replication
publication
Evidence: moderate
Author Information
Author(s): Yang Su Jung, Dennis E Hruby
Primary Institution: Oregon State University
Hypothesis
The A12L protein and its proteolysis are essential for viral replication and morphogenic transition in vaccinia virus.
Conclusion
The A12L protein and its cleavage at the AG/A site are crucial for the production of infectious vaccinia virus progeny.
Supporting Evidence
- The absence of A12L resulted in an 80% reduction in virus replication.
- A12L proteolysis is essential for the formation of mature virions.
- Electron microscopy showed aberrant virus particles in the absence of A12L.
Takeaway
The A12L protein helps the virus make copies of itself, and if it doesn't get cut properly, the virus can't form correctly.
Methodology
A conditional lethal mutant virus was constructed to regulate A12L expression, and its effects on viral replication were analyzed using T-REx 293 cells.
Digital Object Identifier (DOI)
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