Increased gene sampling strengthens support for higher-level groups within leaf-mining moths and relatives (Lepidoptera: Gracillariidae)
2011

Gene Sampling and Phylogenetic Support in Leaf-Mining Moths

Sample size: 57 publication Evidence: high

Author Information

Author(s): Kawahara Akito Y, Ohshima Issei, Kawakita Atsushi, Regier Jerome C, Mitter Charles, Cummings Michael P, Davis Donald R, Wagner David L, De Prins Jurate, Lopez-Vaamonde Carlos

Primary Institution: University of Maryland

Hypothesis

Can adding genes for a subset of taxa improve branch support for deep nodes in the phylogeny of Gracillariidae?

Conclusion

Adding more genes significantly improved support for deep divergences in the phylogeny of leaf-mining moths.

Supporting Evidence

  • Partial augmentation of genes led to high support for deep divergences.
  • More than three-quarters of nodes were supported with bootstrap values greater than 80%.
  • Gracillariidae were monophyletic in all analyses conducted.
  • Strong evidence for the exclusion of Douglasiidae from Gracillarioidea.

Takeaway

The more genes you look at, the better you can understand how different moths are related to each other.

Methodology

The study sequenced 21 nuclear protein-coding genes for 57 taxa and analyzed different data sets to assess phylogenetic relationships.

Potential Biases

Potential biases due to compositional heterogeneity affecting phylogenetic inference.

Limitations

The study faced challenges with compositional heterogeneity and the effects of missing data.

Participant Demographics

The study included 45 species of Gracillarioidea, primarily Gracillariidae.

Statistical Information

P-Value

p<0.015

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2148-11-182

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