Genome of Wheat Stripe Rust Fungus Revealed
Author Information
Author(s): Dario Cantu, Manjula Govindarajulu, Alex Kozik, Meinan Wang, Xianming Chen, Kenji K. Kojima, Jerzy Jurka, Richard W. Michelmore, Jorge Dubcovsky
Primary Institution: University of California Davis
Hypothesis
Next Generation Sequencing can provide rapid access to the genome of Puccinia striiformis f. sp. tritici.
Conclusion
The assembly of the PST-130 genome provides valuable resources for identifying and cloning PST genes and their regulatory regions.
Supporting Evidence
- The genome assembly provides an estimated coverage of at least 88% of the PST genes.
- Comparative genomics approaches were used to improve gene annotation.
- Over 140 families of transposable elements were identified in the PST-130 genome.
Takeaway
Scientists sequenced the genome of a fungus that causes wheat stripe rust, which helps in understanding how to fight this disease.
Methodology
Illumina sequencing was used to obtain nearly 80 million high-quality paired-end reads, which were assembled into contigs.
Limitations
The automatic gene prediction has limitations and requires further curation.
Digital Object Identifier (DOI)
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