Effects of Morphine on Circadian Clock Gene in Mice
Author Information
Author(s): Wang Xiaojia, Wang Yueqi, Xin Haoyang, Liu Yanyou, Wang Yuhui, Zheng Hang, Jiang Zhou, Wan Chaomin, Wang Zhengrong, Ding Jian M
Primary Institution: West China Medical Center, Sichuan University, Chengdu, Sichuan, China
Hypothesis
Does morphine treatment alter the expression of the circadian clock gene mPer1 in mouse brain and kidney?
Conclusion
Morphine treatment alters the circadian rhythm of mPer1 expression in the brain and abolishes it in the kidneys.
Supporting Evidence
- The mPER1 protein exhibited robust circadian rhythmicity in the brains of control mice.
- Morphine administration abolished the circadian rhythm of mPER1 in the kidneys.
- The phase of mPER1 expression in the brains of morphine-dependent mice was significantly advanced compared to control mice.
Takeaway
Morphine changes how a gene that helps control body rhythms works in the brain and stops it from working in the kidneys.
Methodology
The study used male BALB/C mice and involved conditioned place preference tests, Western blot analysis, and immunohistochemistry.
Potential Biases
Potential bias in animal selection and handling.
Limitations
The study was limited to male mice and may not generalize to other populations.
Participant Demographics
Male BALB/C mice, 4–6 weeks old.
Statistical Information
P-Value
< 0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website