Discordant results between radioligand and immunohistochemical assays for steroid receptors in breast carcinoma
1990

Discordant Results in Breast Cancer Receptor Testing

Sample size: 179 publication Evidence: moderate

Author Information

Author(s): H. Helin, J. Isola, M. Helle, T. Koivula

Primary Institution: University of Tampere

Hypothesis

What are the reasons for discordant results between radioligand and immunohistochemical assays for steroid receptors in breast carcinoma?

Conclusion

The study found that discordant results between two types of receptor assays were common, particularly in premenopausal women and when receptor concentrations were low.

Supporting Evidence

  • 18% of ER assays and 30% of PR assays showed discordant results.
  • Immunohistochemistry-positive and steroid-binding-negative results were more common in discordant ER assays.
  • Low binding affinity was significantly associated with discordant assay results.

Takeaway

This study looked at breast cancer tests and found that sometimes the results don't match up, especially in younger women. It helps doctors understand why this happens.

Methodology

The study analyzed surgical biopsy specimens using both steroid-binding and immunohistochemical assays to compare receptor statuses.

Potential Biases

Subjectivity in interpreting immunohistochemical results may introduce bias.

Limitations

The study did not account for all potential factors influencing assay results, such as tumor heterogeneity.

Participant Demographics

Median age of participants was 61 years, with 58 premenopausal and 121 postmenopausal women.

Statistical Information

P-Value

p<0.03

Confidence Interval

95% confidence limits

Statistical Significance

p<0.05

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