Effects of Hypoxia on Very Small Embryonic-Like Stem Cells
Author Information
Author(s): Gharib Sina A, Khalyfa Abdelnaby, Kucia Magdalena J, Dayyat Ehab A, Kim Jinkwan, Clair Heather B, Gozal David
Primary Institution: University of Washington
Hypothesis
Exposing animals to hypoxia will mobilize VSELs from their BM to peripheral circulation in a stem-cell specific chemokine gradient, and activate a spectrum of transcriptional programs.
Conclusion
Exposure to hypoxia recruits pluripotent stem cells to the peripheral circulation and activates diverse transcriptional programs orchestrated by key genes.
Supporting Evidence
- Hypoxia mobilizes VSELs from bone marrow to peripheral blood.
- A distinct genome-wide transcriptional signature is induced in VSELs by hypoxia.
- Chemokine levels increase in response to hypoxia, promoting VSEL mobilization.
Takeaway
When mice are exposed to low oxygen, special stem cells move from their home in the bone marrow to the blood, and they start working on important tasks in the body.
Methodology
The study used an in vivo murine model to investigate the effects of hypoxia on VSELs through gene expression profiling and chemokine level measurement.
Limitations
The study only explored the effects of hypoxia on gene expression and did not assess post-transcriptional modifications or the long-term consequences of chronic oxygen deprivation.
Participant Demographics
Eight-week-old, adult, male, C57BL/J6 mice.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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