Using Shewanella oneidensis to Produce Hydrogen from Algal Enzyme
Author Information
Author(s): Sybirna Kateryna, Antoine Tatiana, Lindberg Pia, Fourmond Vincent, Rousset Marc, Méjean Vincent, Bottin Hervé
Primary Institution: CEA, DSV, iBiTec-S, SB2SM, LPB (URA CNRS 2096), Gif-sur-Yvette cedex, France
Hypothesis
Can Shewanella oneidensis serve as an efficient system for the expression and maturation of the [Fe-Fe] hydrogenase from Chlamydomonas reinhardtii?
Conclusion
The study developed an efficient system for the heterologous expression and maturation of the hydrogenase from Chlamydomonas reinhardtii using Shewanella oneidensis.
Supporting Evidence
- Hydrogen formation by S. oneidensis was significantly increased when grown with specific substrates.
- The system produced 0.4 – 0.5 mg l-1 of catalytically active HydA1.
- HydA1 contained six iron atoms per molecule of protein, confirming its maturation.
Takeaway
Scientists found a new way to make a special enzyme that helps produce hydrogen using a type of bacteria, which is better than previous methods.
Methodology
The study involved using Shewanella oneidensis to express and purify the hydrogenase from Chlamydomonas reinhardtii under anaerobic conditions with various substrates.
Digital Object Identifier (DOI)
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