S100A Expression in Corneal and Cancer Cells
Author Information
Author(s): Li Jing, Riau Andri K., Setiawan Melina, Mehta Jodhbir S., Ti Seng-Ei, Tong Louis, Tan Donald T.H., Beuerman Roger W.
Primary Institution: Xinhua Hospital, Shanghai Jiao Tong University, Shanghai, China
Hypothesis
The study aims to investigate the expression and distribution of S100A genes and proteins in normal corneal-limbal epithelium and ocular surface squamous cell carcinoma (SCC) tissue.
Conclusion
Normal corneal and limbal epithelial cells express a variety of S100 genes and proteins, while ocular surface SCC shows high levels of S100A2, S100A10, S100A8, and S100A9 proteins.
Supporting Evidence
- Twelve S100 mRNAs were identified in human corneal and limbal epithelial cells.
- S100A2 and S100A10 proteins were enriched in basal limbal epithelial cells of normal tissue.
- SCC tissue showed high levels of S100A2, S100A10, S100A8, and S100A9 proteins.
- Expression of S100A2 and S100A10 decreased dramatically in cultured limbal epithelial cells with increased passaging.
Takeaway
This study looked at special proteins called S100A in eye cells, finding that they are important for normal eye function and are also present in cancer cells.
Methodology
The study involved obtaining normal corneal-limbal tissue and SCC tissues, measuring S100A mRNA expression using quantitative PCR, and determining protein distribution through immunofluorescent staining.
Limitations
The corneal epithelium of cadaver tissue was often damaged, which may affect the results.
Participant Demographics
Two patients, both 60-year-old Chinese males, with ocular surface SCC.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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